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DNA 3D Printer Can Allow Scientists To Overnight Synthetic Genes
Jun 25, 2018

As the company ordering custom gene, synthetic DNA is a growing business, so they can in microbial VAT in the production of drugs, industrial enzymes or useful chemicals.Laboratories around the world buy synthetic gene into plant or animal or try new CRISPR based disease treatment.Some scientists even offered to store information in DNA, because a gram of DNA in theory can store the equivalent of 50 million DVDS, and should be stable for hundreds of years.However, this means that the synthesis of DNA than the use of DNA in biotech industry much bigger quantity.

However, the current of the development of DNA synthesis using 1981 based on the methodology of organic chemistry technology to produce about 200 bases of so-called long oligonucleotides, because with the increase of the length, the inevitable errors in the process of the right sequence of lower productivity.To assemble a small gene, scientists must it is presented.the synthesis, long about 200 bases, and then they stitched together.This technique is very time-consuming and very expensive, and is not entirely accurate.

Combined biological energy research institute, Sebastian Palluk and Daniel Arlow were is a member                                              of the Lawrence Berkeley national laboratory

New method of 3 d printers "DNA" based on the discovery of DNA in the cells of the immune system synthetase and TdT (deoxyribonucleotide transferase) at the end, its natural to nucleotide added to the existing DNA molecules in the water, in which DNA is the most stable.Unlike other enzymes, the TdT does not depend on the existing DNA template to reproduce.On the contrary, it will add nucleotide randomly to the preparation for antibodies of the immune system genes.

Ard colleagues Sebastian Palluk said that the TdT in all four aspects of DNA nucleotides effect also good, no side effects, may lead to molecular agglomeration, and very fast, around 200 per minute stretches of DNA bases, if you let it fly free.

In fact, the Berkeley researchers in their first trials show that they are faster, simpler technology in the current technology almost in every step of the synthesis are accurate.

"When we use NGS analysis products, we can determine about 80% of the molecules have expected 10 base sequence," said Arlow were."This means that each step of the production of about 98% on average, for the problem of more than 50 years ago is not too bad, we hope that over 99.9% of DNA".

Once they reach 99.9% of fidelity, they can be a one-off synthesis 1000 bases long molecules, the yield more than 35%, this is the chemical synthesis technology is completely impossible, Palluk said.

This new method aims to accelerate research in many areas, make it easier for biological engineers quickly find out how to make useful products.It may be an improvement in the development of synthetic biology, synthetic biology is a study for the practical purposes of artificial created branch of microorganisms.